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Description
Human TREM2 ELISA KitProduct Specification protein TREM2 Usage 1. Self brought consumables: 1 , plate reader (capable of measuring 450 nm Absorbance at) 2 , multi channel pipette or automatic plate washer 3 , incubator, cryogenic centrifuge 4 Adjustable pipette gun and gun tip 5 Deionized water 2. Reagent preparation: Standard Diluent Ready to use; Before use, equilibrate to room temperature; 2 8 Save. Standard Diluent Used to dilute standard. 1Assay Buffer Before use,
Product Specification
| protein | TREM2 | ||||||||||||||||||||||||||||||||||||||||
| Usage |
1. Self-brought consumables: 1 , plate reader (capable of measuring 450 nm Absorbance at) 2 , multi-channel pipette or automatic plate washer 3 , incubator, cryogenic centrifuge 4 Adjustable pipette gun and gun tip 5 Deionized water 2. Reagent preparation: Standard Diluent ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Save. Standard Diluent Used to dilute standard. 1×Assay Buffer ： Before use, equilibrate to room temperature and press with deionized water 1 ： 10 Proportional dilution of Assay Buffer (10×) get 1×Assay Buffer , mix gently to avoid foaming. 2-8℃ Preservation, this solution can be stable 30 Heaven. 1×Assay Buffer For diluting serum and plasma samples, HRP-conjugated Human TREM2 Detect Antibody (100×) 。 Human TREM2 Standard ： The label superscript added to the standard focuses on suspension volume reconstitution in deionized water Human TREM2 Standard get 20 ng/mL Standards. Before performing dilution, hold the standard at least 15 min And gently shake, after reconstitution Standard Available at -20℃ Save 1 Months, redissolved and used 1 Discard after times. 1×HRP-conjugated Human TREM2 Detect Antibody ： Mix thoroughly before dilution. Depending on the amount required for standard and sample, use in clean plastic tubes 1×Assay Buffer Right HRP-conjugated Human TREM2 Detect Antibody (100×) Conduct 1:100 Dilution. 1×HRP-conjugated Human TREM2 Detect Antibody Should be after dilution 30 min Internal use. HRP Substrate (TMB) ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Store in the dark from light. Stop Solution ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Save. 1×Wash Buffer: Equilibrate to room temperature before use, Wash Buffer (20×) Performed with deionized water 1:20 Dilution to obtain 1×Wash Buffer 。 Mix gently to avoid blistering, store at room temperature, this solution is stable 30 Heaven. Standard curve settings: As shown in the table below, use Standard Diluent Will 20 ng/mL The standard was diluted to 10 、 5 、 2.5 、 1.25 、 0.63 、 0.31 、 0.16 And 0 ng/mL Of Human TREM2 Standards.
Note: Each experiment requires a new formulation of standards. 3. Sample preparation: 1 Cell culture supernatant: centrifuge to remove particles, immediately measure or dispense -20℃ Store and avoid repeated freezing and thawing. 2 Serum: Use a serum separation tube and allow the sample to coagulate at room temperature 30 min , then 1,000 g Centrifugation 15 min 。 Take the upper layer serum for immediate determination or dispensing -20℃ Store and avoid repeated freezing and thawing. 3 Plasma: use EDTA , heparin or citrate as an anticoagulant. After collection 30 min Inner 1,000 g Centrifugation 15 min 。 Immediate determination or dispensing -20℃ Store and avoid repeated freezing and thawing. CAUTION: Do not use specimens with severe hemolysis or lipidemia. If the sample is to be in 24 h Used within, you can store it in 2 To 8℃ , avoid repeated freezing and thawing. Frozen samples should be slowly warmed to room temperature and gently mixed prior to determination. 4. Experimental steps: 1 Remove the excess slats from the plate frame, place them back in the aluminum foil bag containing the desiccant pack, and reseal them. 2 The liquid in each well was removed and washed. Add per well using a multi-channel pipette or automatic washer 300 μL 1×Wash buffer Let stand and soak 30 s , performing washing. Complete removal of the liquid in each step is essential to obtain good performance. After the last wash, the plate was inverted and blotted dry with a clean paper towel to remove any remaining 1×Wash Buffer 。 Use the plate immediately after the plate wash is completed and do not allow the plate to dry. 3 , Add standard: add standard well 100 μL Diluted standards. 4 Add sample: serum / Plasma: sample well addition 80 μL 1×Assay Buffer And 20 μL Pre-diluted samples; Cell culture supernatant: sample well addition 100 μL Cell culture supernatant (See sample preparation for details). 5 , add detection resistance, add each well 50 μL 1×HRP-conjugated Human TREM2 Detect Antibody , guarantee step 3 、 4 、 5 Add samples continuously without interruption. The sample loading process is 15 min Within completion. Plate was coated and incubated at room temperature 2 h 。 6 Repeat steps 2 Washing in 6 Times. 7 , adding per well 100 μL HRP Substrate (TMB) 。 The plate was coated and incubated at room temperature protected from light 5-30 min 。 8 , adding per well 100 μL Stop Solution ， Stop Solution Should be in accordance with the TMB The same order is added to the plate. The color in the hole should change from blue to yellow. If the color in the hole is green or the color changes unevenly, tap the plate lightly to ensure thorough mixing. 9 , in 30 min Within, measure each well 450 nm The absorbance value at. Note: 5-30 min The color development time is within the empirical range. For each specific experiment, the approximate color development time can be determined according to the following conditions. （ 1 ) Visual observation: standard song S5 The holes are light blue, Blank When the hole has no obvious blue color, it can be terminated; （ 2 ) Instrument judgment: 630 nm At left and right wavelengths, standard curve S1 Porous OD Value reaches 0.5 - 0.7 、 S5 Porous OD Value reaches 0.05 - 0.08 、 Blank Porous OD Value less than 0.05 It can be terminated. 5. Result calculation: 1 Calculate the multiwell average of each standard and sample OD Value, and subtract the zero concentration ( Std.8 ) average OD Value. 2 , Drawing of standard curve: take the standard concentration as x Axis, the average absorbance of each standard is y Axis, plot the standard curve. You can use mapping software to create standard curves. Note: If the sample is diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 6. Results display: Typical standard curve ( R2≥0.99 ） Figure1. 96 Well plate analytical Human TREM2 Standard curve. The data and curves are for reference only, and experimenters need to establish standard curves according to their own experiments. |
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| Sensitivity | 0.16 ng/mL | ||||||||||||||||||||||||||||||||||||||||
| Species Reactivity | Human | ||||||||||||||||||||||||||||||||||||||||
| Source | Human | ||||||||||||||||||||||||||||||||||||||||
| Synonym | Human TREM-2 ELISA Kit | ||||||||||||||||||||||||||||||||||||||||
| Detection Type | Sandwich method | ||||||||||||||||||||||||||||||||||||||||
| Description | Myeloid cell trigger receptor 2 (TREM2) is a single Ig domain receptor that is expressed in macrophages and dendritic cells, but not in granulocytes or monocytes. It is most abundantly expressed in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, while microglia in the central nervous system are the most dominant cell type producing TREM2. TREM2 plays a role in chronic inflammation and can stimulate the production of constituent rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP to activate the immune response of macrophages and dendritic cells. It is also associated with the adaptor protein DAP12 to transmit activation signals, which play a role in innate and adaptive immune responses. TREM2 Signaling is also an important way to promote colonic wound healing. TREM2 mutations increase the risk of neurodegenerative diseases such as Alzheimer's disease, amyotrophic lateral sclerosis, and Parkinson's disease. The Human Myeloid Cell Trigger Receptor 2 ELISA Quantification Kit employs a double antibody sandwich method to quantify human myeloid cell trigger receptor 2 in a sample. The plate is coated with an antibody specific to human myeloid cell trigger receptor 2, and the sample or standard and the specific detection antibody labeled with HRP are added to the wells of the plate. The human myeloid cell trigger receptor 2 binds to the solid phase antibody and the detection antibody coated on the plate. After washing, a chromogenic substrate (TMB) was added, and the TMB became blue under HRP catalysis and turned yellow after addition of the stop solution. The OD value was measured with a microplate reader at 450 nm wavelength, and the concentration of human myeloid cell trigger receptor 2 was directly proportional to the OD450 value. |
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| Composition |
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| General Notes | 1. If Standard Diluent and Assay Buffer (10 ×) turn yellow in color and have a small amount of precipitation, it is because the reagent contains serum. Just centrifuge to remove the precipitation, which will not affect normal use. 2. Do not mix components with different batch numbers and different manufacturers; Failure to do so may lead to abnormal results. 3. Change the tip frequently to avoid cross-contamination between the components. 4. To ensure accurate results, the plate must be properly adhered during the incubation step. 5. Stop Solution is corrosive to a certain extent, so please take protective measures when operating. |
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| Instructions | Serum (plasma), cell culture supernatant | ||||||||||||||||||||||||||||||||||||||||
| Storage Temp. | 2-8 ℃, protected from light, valid for 24 months. | ||||||||||||||||||||||||||||||||||||||||
| Test Range | 0.16 ng/mL-10 ng/mL |
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4.4 ★★★★★
Based on 1071 reviews
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Product Reviews
★★★★★ 5
True to size & Use a Drill
Color: Beige, Size: 3 Panel-102‘’-Round Pad, Color: Beige, Size: 3 Panel-102‘’-Round Pad
Love this! Works perfectly for where I wanted it. I did measure my room incorrectly and thought it would be smaller, thankfully it’s not and thankfully it’s not bigger than the room itself. I did like how the whole thing was move-able but it was a bit annoying so I just added extra clips to the left of the middle one and unscrewed the right of the middle at the bottom so it opens like a door, this did make it a bit flimsy but duck taping it to the wall as anchor it works.
100% recommend, but do suggest a drill and a second set of hands to put it together, due to its size it can be a bit difficult to maneuver putting together yourself.
Also if you have a clingy cat like I do, and think this will help keep them out, no, my cat who is very large can manage to squeeze underneath it.
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Reviewed in the United States on March 9, 2024
★★★★★ 1
waste of money
Color: Black, Size: 4 Panel-136‘’W-Round Pad
This is a total waste of money. Its only going to free stand if you arent moving it. If you are planning on dividing a room, and then folding these for space and then dividing the room again, itll last a few days. The poles kept coming off of the screws that holds the fabric on the poles together. Was fixing this EVERY DAY. Finally got tired of doing this and just threw it out. Lot of money spent on garbage. Get a sheet room divider, or something that isnt cheaply made. Was simply making an office type space in a bedroom, to seperate the two areas for work, and folding it kept making the poles come off those twisted screws. just not worth it.
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Reviewed in the United States on May 3, 2026
★★★★★ 5
Absolutely love this room divider!
Color: Beige, Size: 3 Panel-102‘’-Round Pad, Color: Beige, Size: 3 Panel-102‘’-Round Pad
When they say you can't see anything through it, they are correct. As you can see by the video, the cars are traveling by with the sunlight on the divider and you still can't see any cars passing nor can you see the ones in the parking lot. You can also tell it blocks out the sunlight too which is a definite plus for our store. Quality material on the divider. Material is nice and snug on the poles with no ripples. I also like the base of these, they make the divider nice and sturdy and can be adjusted if floor is uneven. Also the design of the legs allow them to go under your furniture. Also putting them together is pretty easy. Just make sure you when you are putting the poles together to make sure at the top of the pole that the indent screw hole faces out. This makes for a better look. The directions don't say which way to turn and at 1st, I had one, one way and the other was another way as both ways work that way but I wanted a uniform/better look.
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Reviewed in the United States on July 29, 2024
★★★★★ 4
Works well, need power tool to assemble
Color: Beige, Size: 3 Panel-102‘’-Round Pad, Color: Beige, Size: 3 Panel-102‘’-Round Pad
Sturdy enough, especially when feet are positioned for balance. Works perfectly to create a WFH “cubicle”. Can adjust the size to provide complete privacy or fold back a panel when I want more openness. The fabric panels are not perfectly taught, but pretty close. The only negative is that without my husband’s power drill/screwdriver we wouldn’t have been able to put it together. It would take the strength of Thor to do it with just a manual screwdriver.
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Reviewed in the United States on November 30, 2022
★★★★★ 5
Now I don't have to see the "black hole" that used to be my family room!
Color: Black, Size: 4 Panel-136‘’W-Round Pad
I have an adult child living at home and his bedroom is tiny, so he's pretty much taken over the family room. We are both much happier now that the divider is up. It is about 12 feet long and gives him privacy to watch his movies and play his video games. And I don't have to see all the shoot-em-up stuff he likes to watch. I put the divider together with just a screwdriver (not a power one, that would be nice but I don't have so I had to use a little elbow grease.) It took a couple hours. The instructions are straightforward. I did have to use my long dining room table to attach the feet to the divider panels, which was a bit tricky. But I'm really pleased with the end result. I'm NOT a handywoman, so I feel proud that I did it "all by myself." He wanted the black fabric, but I think the lighter colors would have been better for a room that gets hot in the summer. Just my opinion. Whatever color you choose, I think you'll like the end result.
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Reviewed in the United States on July 18, 2023