SKU: 32645012309

UA-Glo Luminescent Mycoplasma Detection Kit

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Description

UA-Glo Luminescent Mycoplasma Detection KitProduct Specification Synonyms Mycoplasma luciferase detection kits Stability & Storage Dry ice transport. 20 storage is protected from light, and the validity period is 12 months Background The UA Glo Mycoplasma Detection Kit is used for the qualitative detection of mycoplasma contamination in cell cultures. Over 95% of mycoplasma contamination in cell cultures is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M. hominis, M.

Product Specification


Synonyms Mycoplasma luciferase detection kits
Stability & Storage

Dry ice transport. -20℃ storage is protected from light, and the validity period is 12 months

Background

The UA-Glo Mycoplasma Detection Kit is used for the qualitative detection of mycoplasma contamination in cell cultures. Over 95% of mycoplasma contamination in cell cultures is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M. hominis, M. salivarius, M. arginine, and Acholeplasma laidlawii. Live mycoplasmas produce kinases that are not expressed by eukaryotic cells, which can convert ADP into ATP. The Mycoplasma Detection Kit provides ADP and other substrates required for mycoplasma kinases to convert ADP into ATP, and then detects the generated ATP via a luciferase luminescence method to confirm whether there is mycoplasma contamination in the cell culture. This kit can quickly detect the eight aforementioned species as well as many other common live mycoplasma strains.

Components

Size

component

10T

Mycoplasma luciferase detection reagent 0.5ml

Mycoplasma luciferase detection reagent 0.1mL

25T

Mycoplasma luciferase detection reagent 1.25ml

Mycoplasma luciferase detection reagent 0.25mL

50T

Mycoplasma luciferase detection reagent 2.5ml

Mycoplasma luciferase detection reagent 0.5mL


Protocol

1. Sample Preparation for Cell Culture Medium to be Tested

  1. Take 1 mL of cell culture medium from cells in the logarithmic growth phase.

  2. Centrifuge the cell culture medium at room temperature (200 g) for 5 minutes. Transfer approximately 800 μL of the supernatant to a new centrifuge tube, making sure not to include any cell debris at the bottom.

  3. Use the prepared cell culture medium for the mycoplasma detection with luciferase assay.

2. Luciferase Mycoplasma Detection

  1. Add 50 μL of the cell culture medium sample to a white opaque 96-well plate.

  2. Equilibrate the luciferase mycoplasma detection reagent and substrate to room temperature (22°C-25°C), and mix gently.

  3. Add 50 μL of the luciferase mycoplasma detection reagent to the 50 μL of the cell culture medium sample.

  4. Mix by gentle shaking for 10 seconds, incubate at room temperature for 5 minutes, and then read the fluorescence value (Reading 1).

  5. Add 10 μL of the luciferase mycoplasma detection substrate.

  6. Mix by gentle shaking for 10 seconds, incubate at room temperature for 10 minutes, and then read the fluorescence value (Reading 2).

3. Results
The ratio of Reading 2 to Reading 1 is used to determine if mycoplasma contamination is present.

 

The ratio of reading 2 to reading 1

Results Explanation and Recommendations

< 1

No Mycoplasma contamination

1-1.2

Possible Mycoplasma contamination. The cells were isolated and tested again after culture for 24-48hr.

> 1.2

Mycoplasma contamination



Guidelines

  • Aliquot Storage: Aliquot and store the reagents according to the instructions at -20°C to ensure reagent stability.

  • Freeze-Thaw Stability: Reagents stored at -20°C, protected from light, can be freeze-thawed twice without affecting the test results.

  • Sample Collection: Collect samples from cells in the logarithmic growth phase for testing. Newly passaged cells should be tested 24-48 hours after subculture.

  • Luciferase Reaction Sensitivity: The luciferase reaction is sensitive to temperature changes. Reagents and samples should be equilibrated to room temperature (22°C-25°C), and the temperature should remain constant (±1°C) throughout the test.

  • For Research Use Only: This product is intended for research purposes only.

Shipping Notes
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Exchange/Return Notes
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SKU: 32645012309

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4.3 ★★★★★
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Verified Purchase
Arsenio Braga
Houston, US
★★★★★ 5
There is a seam up the front. I think perhaps it being seamless would be better.
Size: Medium, Color: Black
Like anything off Amazon I wasnt expecting anything perfect and ready to have to mail it back. Pleasantly surprised by the softness and durability. The waist band isn't to small on a medium so it fits actually so comfy. I wear them as underwear and as shorts for my skirts n dresses. Id recommend but I say size up if you want a comfy fit. But down if you want a fitted fit fit. Comfy still is fitted but better feeling on if that makes sense. Also the crotch of the shorts/undies has essentially extra fabric sewed in creating that nice comfort. Ill snag a photo when I have time.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 3, 2026
S
Verified Purchase
Stephanie Vaughn
Whiting, US
★★★★★ 4
Product varies from package to package
Size: X-Large, Color: Black/Navy/Dark Purple/Red
Over a two year period this is my 4th purchase. They started as sleep shorts and have replaced other underwear most of the time because they are comfortable and the quality is pretty good. However there are differences in between every package. The length has varied up to an inch, the material has changed, and some are slightly tighter than others. That said it works for me. I am a side sleeper and sometimes the side seam bothers me.
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Reviewed in the United States on March 9, 2026
H
Verified Purchase
House of Squawk
Phoenix, US
★★★★★ 5
Very comfortable in the Texas heat.
Size: Small, Color: Black/Navy/Dark Purple/Red
I got some of these as a gift and I loved them so much I purchased two more packs. I just opened my new packs and put them in the washer so I'm not leaving a photo. When I wear them they look exactly like they do on the models. I've been doing a lot of heavy outdoor work in the heat and I get drenched. These underwear have a 100% cotton crotch and the rest is 95% cotton and 5% spandex. When I first saw the wide crotch I didn't think I would like it. But these are soooo comfortable. I'm 4'11" and weight 108-112 and the size small fits loosly but well. I could probably get away with an XS but I like this loose and not too tight fit. I see some folks say these roll up. They don't roll up on me. I think they might roll if they were tight fitting. I have lots of other undergarments like these and some roll and some don't. But none of the others are cotton. They are mostly spandex and since I've found these in cotton that's all I'm wearing until summer is over. I can't feel them or see them under pants or skirts. These look more like shorts than underwear and they have a very nice thickness. Since I only had four pair to begin with, those got washed and dryed a lot. I can't tell if they shrunk since I only tried them after washing. Sorry I forgot to compare the size of the new ones to the older ones before I threw them in the washer. I'll probably buy another set and compare at that time. One thing I can report is that they do not fade at all. I just washed the black, purple and red colors with the white color and the white is still completely white. Now I have more colors and I'll probably be walking around in my underwear and no one will know. The only con I can think of is the wide crotch wouldn't work well with pads with wings, in my opinion. Try a size larger for a nice cool pair of sleeping shorts to see how they fit while they are on sale. Then go smaller if needed. I think you might enjoy these.
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Reviewed in the United States on July 10, 2024
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Verified Purchase
Shannon
Draper, US
★★★★★ 5
So comfortable and worth the buy
Size: Large, Color: Black
So comfortable!!! There is no seam in the butt and under neath, only in the front but it is really soft and non irritating. I got these for post partum but I’m already wearing them at 8.5 months pregnant. They’re probably my favorite undies right now. The length is also perfect and the material is very soft and stretchy. The waist band is the same, soft and stretchy.
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Reviewed in the United States on April 11, 2026
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Verified Purchase
Jeanne
San Leandro, US
★★★★★ 3
Cotton Blend Was Just Too thick
Size: 3X-Large, Color: Black
I was surprised by how thick these boxers were, thicker than my jeans, much thicker than my PJs! Thick enough that I could wear them outside the home without jeans. In spite of the thickness, they were soft and very comfortable. The 3XL is a perfect fit on my 5’2” apple shaped, short waisted 215 lb body. The boxer cut helped prevent chafing, a major plus. Menstrual pads, with or without wings, do not adhere to this material, a major minus. Ultimately, feeling like I was wearing jeans to bed or wearing two pairs of pants outside the home, was just too uncomfortable for me. So disappointed because these boxers were such a great and comfortable fit.
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Reviewed in the United States on April 4, 2026

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