SKU: 95380287749

UA-Glo® Luminescent Kinase Assay

Sale price$74.70 Regular price$83.00
Save 10%

Pay in installments of $20.75 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 22 - Jul 27

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

UA-Glo® Luminescent Kinase AssayProduct Specification Stability & Storage Dry ice transportation. Store in the dark at 20C or below, with a validity period of 12 months. Background The UA Glo Kinase Luminescent Detection Kit can quantitatively determine the activity of kinases in enzymology experiments. This kit measures kinase activity by quantitatively detecting the amount of remaining ATP after the kinase reaction: the amount of remaining ATP is negatively correlated with kinase

Product Specification


Stability & Storage

Dry ice transportation. Store in the dark at -20°C or below, with a validity period of 12 months.

Background

The UA-Glo Kinase Luminescent Detection Kit can quantitatively determine the activity of kinases in enzymology experiments. This kit measures kinase activity by quantitatively detecting the amount of remaining ATP after the kinase reaction: the amount of remaining ATP is negatively correlated with kinase activity. When using this kit, the maximum ATP concentration in the kinase reaction can reach 10μM; when using the UA-Glo Kinase Plus Luminescent Detection Kit, the maximum ATP concentration in the kinase reaction can reach 100μM; and when using the UA-Glo Kinase Max Luminescent Detection Kit, the maximum ATP concentration in the kinase reaction can reach 500μM. This kit can detect most kinases, and kinase substrates include polypeptides, proteins, lipids, or sugars, etc. The kit can be used for high-throughput homogeneous screening of kinase inhibitors, and can distinguish between ATP-competitive and non-competitive inhibitors by adjusting the ATP concentration.

Components

Product composition: Luciferase, luciferin, and buffer are mixed and filled into 10 ml or 100 ml brown bottles. The specifications are as follows:

Specification

96-well plate reaction count

384-well plate reaction count

10 ml

200

1,000

100 ml

2,000

10,000

10 x 100 ml

20,000

100,000

Protocol

1. Kinase Reaction
1)Perform the kinase reaction in a 96-well or 384-well white opaque assay plate. It is recommended that the reaction volume is 50μL for 96-well plates and 10μL for 384-well plates. Test compounds with concentration gradients can be added to the kinase reaction.
2) The concentrations of kinase and substrate in the kinase reaction need to be optimized according to different kinases. Under the condition of the appropriate signal-to-noise ratio required for the experiment, the kinase concentration within the linear reaction range of the signal can be used for the experiment.
3) Concentration of ATP: The UA-Glo Kinase Luminescent Detection Kit can be used up to 10μM.
4) The kinase reaction can be carried out in a universal reaction buffer (40mM Tris-HCl (pH 7.5), 0.1 mg/ml BSA, 20mM MgCl2) or using reaction buffers and cofactors reported in the literature.
5) The temperature and time of the kinase reaction need to be set according to different kinases. For high-throughput screening experiments of compounds, it is recommended to optimize the kinase reaction to be carried out at room temperature (22℃-25℃) to help maintain the temperature uniformity of the assay plate during the kinase activity determination.

2.  Kinase Activity
1) Take out the UA-Glo Kinase Luminescent Detection Reagent and equilibrate it to room temperature. Mix gently by shaking. The luciferase reaction in the kinase activity luminescent detection reagent is sensitive to temperature changes; the reagent and assay plate need to be equilibrated to room temperature, and the temperature should be kept constant (±1℃) during the test.
2) If the kinase reaction is carried out at a non-room temperature, such as 30℃, the assay plate needs to be equilibrated to room temperature.
3) Add 50 µL of kinase luminescent detection reagent to a 50 µL 96-well assay plate, or 10 µL of kinase luminescent detection reagent to a 10 µL 384-well assay plate, shake to mix, and place in the dark for 10 minutes.
4) Read the fluorescence signal. Since the fluorescence signal is very stable, if necessary, the plate can be read within 3 hours after adding the detection reagent.

Guidelines

1) It is not recommended to arbitrarily change the amount of reaction reagents without strict verification. The volume ratio of the kinase reaction reagent to the UA-Glo kinase luminescent detection reagent should be 1:1.

2) After the first use of the UA-Glo kinase luminescent detection reagent, it should be aliquoted and stored at -20°C to ensure the stability of the reagent. There is no loss of signal intensity after 4 repeated freeze-thaw cycles.

3) This product is for research use only.

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 95380287749

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.1 ★★★★★
Based on 21 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
K
Verified Purchase
Karen
Los Angeles, US
★★★★★ 5
Durable
Color: S6-muti-color
The balls are great for chewers. The squeaky didn’t last long but they are perfect for the ball launcher.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 18, 2026
C
Verified Purchase
Candice K
Grantham, US
★★★★★ 4
The squeeker isn't durable, but the balls themselves are.
Color: S6-muti-color, Color: S6-muti-color
I have two 70+lb, ball obsessed dogs (a field bred Golden Retriever, and an Old Time Scotch Collie), and these balls are living up to the abuse they're receiving. In the fall we always lose a ball or two in the yard due to getting lost in leaves or longer grass, so I always try and buy a multi pack at the start of the season, so went with these this year. I'm glad I did. Great price for a 6 pack, they fit the medium Chuck-It launcher (a little loosely, but they throw just fine), have great bounce, they FLOAT (which means I didn't lose any at the beach), and they're super durable to the dreaded "mouth squish." The squeaker dies pretty quick, like, within a day, but it isn't the kind that can fall out or choke your dog, so no worries there. The boys are happy, I'm happy, my wallet is happy - I've already ordered another pack.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 2, 2024
S
Verified Purchase
S. Berg
Dallas, US
★★★★★ 5
Good balls
Color: S6-glow
My dog is a chewer, and these have held up for close to a year actually a pretty good product
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
N
Verified Purchase
nanny america
Lake Worth, US
★★★★★ 3
Disappointed
Color: S6-muti-color
These were great for about 20 minutes then they stopped squeaking...we have 2 left that I have saved but the other 4 do not squeak anymore...disappointed as my dog loves to squeak things...would not buy these again...My dog is only an 8lb dog too...but he can still play with the balls without the squeak...
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 26, 2026
J
Verified Purchase
joymom
New York, US
★★★★★ 5
Great Value for a ball my dog loves!
Color: S6-muti-color
I wish these ball were in the subscription program! My dog gets sooo excited by the new ball, but her interest in things lasts for a few days. She kills the squeak within 11minutes, but the squeaker stays in place rather than becoming a chocking or swallow hazard, and she still loves to chew it. Next, we must throw the ball approximately 723 times the first day, possibly 496 times on day 2, and just 3- 4 on day 3, at which point she will chase the ball before hollering "it's over here if you need it," while she checks on the chipmunk den. We currently have 17 of these balls in our yard (because we have given several leftovers to a less discriminating doodle next door). These balls hold up really well, get her extremely active for the first couple of days, and are a much cheaper than doggie daycare (after an active morning with a new ball, she is happy to chew on it and sleep much of the day). I always have these on hand!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 22, 2025

recommand products