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Description
Human MMP-9 ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human MMP 9 antibodies were pre coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the MMP 9 present in the sample binds to the solid phase antibody
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human MMP-9 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the MMP-9 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, Gelatinase B, GELB, macrophage gelatinase, MANDP2, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, MMP9, MMP-9, type V collagenase | |||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
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| Background | Matrix metalloproteinases (MMPs), also known as matrixins, are members of the zinc-calcium-dependent proteolytic enzyme family, which can break down the extracellular matrix (ECM) and participate in the processing of various molecules in different subcellular environments. MMPs play important functions in numerous physiological processes, such as embryonic development, morphogenesis, reproduction, and tissue remodeling. Also involved in inflammation as well as autoimmune diseases such as arthritis, cancer and cardiovascular diseases. The amount of newly synthesized MMPs is mainly regulated at the transcriptional level, and the proteolytic activity of already existing MMPs is controlled not only by the activity of the zymogen, but also by the inhibition of enzyme activity by endogenous inhibitors, such as α2-macroglobulin, and tissue inhibitors of metalloproteinases (TIMPs). MMP-9 (also known as gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, type V collagenase) is secreted as a proglycosylation enzyme. Activation of the zymogen involves proteolytic removal of the N-terminal precursor region, resulting in the formation of an active enzyme of 82 kDa. Active human MMP-9 has 72% and 74% amino acid sequence homology with mouse and rat MMP-9, respectively. In addition to the zinc binding site, the catalytic domain contains three consecutive fibronectin type II homologous units that bind to gelatin. A proline-rich hinge region links the catalytic domain to the C-terminal heme-like domain. In vitro experiments, after treatment with 4-aminophenyl ethyl acetate (APMA), the enzymogen can not only produce active enzyme, but also produce a C-terminal truncated form with equivalent activity. MMP-9 can degrade components in ECM, especially with very high specificity for denatured collagen (gelatin). MMP-9 also cleaves collagen types III, IV, V, XI, as well as elastin, nestin-1, and vitronectin. MMP-9 can also cleave a variety of chemokines and growth factors (e.g., IL-1β, CXCL8/IL-8, CXCL7, CXCL4, CXCL1, LatentTGF-β, membrane-bound TNF-α, VEGF, and FGFbasic), beta-amyloid, substance P, myelin basic protein. This action may increase or decrease the biological activity of these soluble factors, and may also cause them to be released from association with the ECM. MMP-9 can also trigger signaling pathways through various membrane proteins or induce them to fall off the cell membrane to inhibit signaling pathways (E.g. CD44, E-cadherin, integrin, ICAM-1 and IL-2Rα). MMP-9 is produced by a variety of normal and transformed cells, including neutrophils, monocytes, macrophages, astrocytes, fibroblasts, osteoclasts, chondrocytes, keratinocytes, endothelial cells, and epithelial cells. It affects physiological and pathological angiogenesis and vascular remodeling. Activated neutrophils release the MMP-9 precursor and do not bind to TIMP-1, allowing pro-angiogenic FGF-2 to be released from the ECM. The complex of MMP-9 and TIMP-1 failed to induce the release of FGF-2. Neutrophil-derived MMP-9 can exacerbate the inflammatory response, inducing the release of additional neutrophil MMP-9 by the production of collagen-derived polypeptides. MMP-9 also plays an important role in bone formation and remodeling, methamphetamine-induced behavioral sensitization and response, regulation of neuronal synaptic remodeling, trophoblast invasion during implantation, and activation of serine protease inhibitor α1. MMP-9-mediated shedding of adhesion molecules also has a direct effect on tumor cell invasion. Circulating levels of MMP-9 are elevated in many inflammatory disorders, including the formation of intraluminal thrombus, atherosclerosis, Crohn's disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy. The ratio of MMP-9 to TIMP-1 was elevated in multiple sclerosis sera and cystic fibrous sputum but decreased in cytomegalovirus-infected sera. The level of free MMP-9 and the level of MMP-9 and lipocalin-2/NGAL complex were elevated in the urine of patients with ovarian cancer and patients with uterine urethral tract infection, respectively. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 31.3pg/mL-2000pg/mL |
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★★★★★ 5
Love This Watch...!
Color: Black/Green
Waiting for a delivery of an expensive diving watch and in need of a watch for my sporting needs, I opted for this as an interim fix. However, it more than filled my need! Nice to look at! (Several compliments received.)
Best part is the watch has not lost even 1 second after 3 weeks on my wrist. Never expected that level of accuracy!!
While the current band suits the watch's appearance, an upgraded band would fool many into thinking it was far more expensive.
Great watch to gift to a buddy...!
I would categorize this as a "Best Buy"!
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Reviewed in the United States on March 18, 2026
★★★★★ 5
Great timepiece!
Color: Silver/Blue
This is a cool watch! I collect many different styles & price points of watches. There is nothing not to like about this watch. The dial, strap & completely luminous dial make it a great time piece. It's a smart looking field watch that lays nicely on the wrist. $20 is an amazing bargain for this watch. Highly recommend!
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Reviewed in the United States on April 19, 2026
★★★★★ 3
Battery life expectancy is below average.
Color: Silver/Green
Love the watch. But now is dead …battery did not last not even 2 years.. look and feel and overall quality is great battery not so much.. now I am hunting for another watch again..
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Reviewed in the United States on March 19, 2026
★★★★★ 5
Good value for money.
Color: Silver/Green
Nice looking watch, very readable, good luminescence, good value for money.
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Reviewed in the United States on February 20, 2026
★★★★★ 5
Nice!
Size: One Size, Color: Black - Magnetic Front Pocket
Nice!
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Reviewed in the United States on May 21, 2026