SKU: 19457208937

Human IL-4 High Sensitivity ELISA Kit

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Description

Human IL-4 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human IL 4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 4 existing in the

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-4 existing in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-4 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-4 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.03pg/ml

Detection Range: 0.25-16 pg/ml

Recovery Range: 94-105%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 4 (IL-4) is a monomeric cytokine of about 18-20kda that can induce naive helper T cells (Th0 cells)Differentiation into Th2 cells. After being activated by IL-4, Th2 cells then secrete IL-4 in a positive feedback manner. The cell type that secretes IL-4 to induce Th0 cell differentiation has not yet been identified, but recent studies have shown that basophils may be this effector cell. IL-4 is closely related to IL-13 and has similar functions.

IL-4 can also promote rhabdomyosarcoma to undergo mitosis, dedifferentiation and metastasis. It can be observed that IL-4 and other Th2 cytokines are involved in airway inflammation in allergic asthma patients.

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SKU: 19457208937

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4.3 ★★★★★
Based on 27 reviews
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Serena R.
Los Angeles, US
★★★★★ 4
Perfect for limited space
Color: Brown, Size: 13"D x 23.7"W x 32.7"H, Color: Brown, Size: 13"D x 23.7"W x 32.7"H
This bar cart works well for small spaces or areas. I got this cart since I live in a 700 sq ft apartment and I needed something that would store all my liquor and equipment, as I barely have any counter space. As seen in the photo, it looks great, too. Was relatively easy to assemble as well. The only complaint I have is the wheels. I seriously considered taking them off as they can be annoying at times and get stuck easily. If it wasn’t for the wheels, I would’ve given the cart 5 stars. Still works great given how inexpensive it is.
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Reviewed in the United States on December 29, 2024
R
Verified Purchase
Ruth White
Dallas, US
★★★★★ 5
Wood bar cart
Color: Brown, Size: 13"D x 17"W x 33"H, Color: Brown, Size: 13"D x 17"W x 33"H
Great piece for my den. Classic look and fits my preppy style well. Easy to assemble.
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Reviewed in the United States on February 24, 2026
M
Verified Purchase
Matthew M. Frick
West Palm Beach, US
★★★★★ 5
Great Bar Cart/Coffee Bar
Color: Brown, Size: 13"D x 23.7"W x 32.7"H
Easy set up, good size, great for decor and holding bottles!
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Reviewed in the United States on January 3, 2026
S
Verified Purchase
SubParChaosCoordinator
Lowell, US
★★★★★ 5
Perfect for the Price!
Color: Greige, Size: 13"D x 23.7"W x 32.7"H
The bar cart was very easy to put together. I can be "mechanically challenged" and was able to do it myself LOL It's nice and sturdy, the wheels roll smooth when you move it. The size is perfect for the small wall I have it against. I have several bottles of liquor and bartending mixer set on top, and about 20 various glassware on the 2nd shelf. The bottom shelf is taller, and does let you stand larger bottles down there. The one racks will only for the smaller size, but does hold them well.
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Reviewed in the United States on November 6, 2024
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Christian
San Leandro, US
★★★★★ 3
Decent bar cart, but very annoying assembly
Color: Marble White, Size: 13"D x 23.7"W x 32.7"H
I think it looks nice and is decent value, but this thing is a pain to put together. Instead of just sending 3 shelves, you get 6 pieces to connect together to make 3 shelves. On top of that - they don’t even really connect. It would be 1000x easier if they added a couple of wooden pegs to connect the 2 pieces together. Instead, you have to do a balancing act which is tedious as can be. I ended up super glueing 2 of the 3 shelves togethers after how annoying the first was, so I would highly recommend you do the same. The whole process of putting it together feels like a balancing act. For something so small, it’s about as annoying as possible to put together. Nearly every step requires you to balance something and screw it in. Most of this would be alleviated if, as I said, it was just 3 pieces for the shelves instead of 6. But, you get what you pay for. Overall, I can’t complain too much for the price and result, but I wasn’t a fan of building it. It’d also look a little nicer if each shelf didn’t have a crack going through it.
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Reviewed in the United States on March 14, 2026

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