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Description
Human TBK1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a TANK Binding Kinase 1 (TBK1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of TANK Binding Kinase 1 (TBK1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human TANK Binding Kinase 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | TANK-binding kinase 1, also known as TBK1, is an enzyme encoded by the TBK1 gene. It is an enzyme with kinase activity. Specifically, it is a serine/threonine protein kinase. This kinase is primarily known for its role in innate immune antiviral responses. However, it also regulates cell proliferation, apoptosis, autophagy, and anti-tumor immunity. Inadequate regulation of its activity can lead to autoimmunity, neurodegenerative diseases, or tumorigenesis. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 1429 reviews
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Product Reviews
★★★★★ 5
Dermatologist-Recommended, Gentle Coverage with Added Sun Protection
Size: 1.7 Fl Oz (Pack of 1), Size: 1.7 Fl Oz (Pack of 1)
This Eucerin Tinted Moisturizer was recommended to me by my dermatologist after having MOHS surgery performed on my face twice. At that point, sun protection became non-negotiable. He felt that the moisturizer with SPF 30 that was already part of my skincare routine was not sufficient on its own and suggested adding this product for better daily protection.
I’m so glad I followed his advice. This tinted moisturizer provides lightweight, natural-looking coverage that evens out my skin tone without feeling heavy or settling into fine lines. It blends easily and leaves my skin looking healthy and polished, not made-up.
What I appreciate most is how hydrating and gentle it is. My skin can be sensitive, especially after procedures, and this has caused no irritation or breakouts. It wears comfortably all day and layers well with other skincare products when needed.
The coverage is sheer, so it won’t replace foundation if you want full coverage, but it’s perfect for everyday wear and gives me the confidence to step out with minimal makeup while knowing my skin is protected.
I would highly recommend this to anyone who has sensitive skin, a history of skin cancer or procedures, or who simply wants reliable sun protection with a natural finish.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 24, 2026
★★★★★ 5
The Lazy Makeup Day MVP
Size: 1.7 Fl Oz (Pack of 1)
This has become my easy low-maintenance face day product. It gives just enough tint to even things out while still giving solid SPF coverage, so I can skip foundation without feeling totally bare-faced. The finish feels lightweight, my skin stays comfortable all day, and the color blended into my skin way better than I expected from a “universal” tint.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 6, 2026
★★★★★ 4
Overall good aesthetic result for mineral sunscreen, but some downsides
Size: 1.7 Fl Oz (Pack of 1)
It is quite good overall, it blends in perfectly to my medium-light warm tone skin. There is no white tint. It seems effective in terms of SFP and doesn't leave a greasy or heavy feel, ends up looking lightly glowy but reasonably dry to the touch. I'm not sure you'd want to apply it thick enough to substitute for foundation.
What I don't like
- Like makeup the color can rub off on clothes etc.
- Doesn't seem to reapply very well, leads to pilling, and also rubbing my face at all leads to pilling. It's very "don't touch it again after you applied once"
- Quite hard to remove with normal cleanser, I think I might need to remove it with oil or something?
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Reviewed in the United States on January 29, 2026
★★★★★ 3
Way too heavy & greasy for combination skin
Size: 1.7 Fl Oz (Pack of 1)
Ugh this tinted sunscreen is such a let-down. I was excited to find an option that's a mineral sunscreen, with tinting that's supposed to work for all skin tones, is made with non-comedogenic ingredients, and is a more reasonable price than what my old tinted sunscreen now costs. I saw mixed reviews on the greasiness / heaviness of this sunscreen, but decided to give it a try, especially because I have combo skin that gets pretty dry in the winter... but wish I had listened to the people who said this is overwhelmingly thick.
Pros: the tint works fairly well for my light-to-medium warm-toned skin (although you can see in my video it doesn't blend at all well with my hand's skin tone... so can't imagine this will be universal), it doesn't irritate my combination skin that can be very sensitive, and hasn't blocked my pores.
Cons: This feels like I'm smearing peanut butter on my face - it is such a thick and oily consistency. I have combination skin, with some areas that are especially dry right now in a northern winter climate... but this is not what I would call "moisturizing" - it's greasiness that is impossible to rub in, doesn’t dry down, and instead just sits on the skin (and I'm not even using close to the amount of sunscreen you're supposed to in order to get the true SPF coverage). I use tinted sunscreen to replace the need for a BB cream or foundation, and this unfortunately would never be appropriate for everyday wear.
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Reviewed in the United States on February 27, 2026
★★★★★ 5
Not super greasy, thankfully!
Size: 1.7 Fl Oz (Pack of 1)
Very gopd product! Goes on smooth and protects well. Non greasy feel.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 20, 2026
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