SKU: 81588829602

Rat IFN-γ ELISA KIT

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Description

Rat IFN-γ ELISA KITProduct Specification Description Detection Principle: The double antibody sandwich ELISA method was used in this experiment. Anti rat IFN The monoclonal antibody was coated on the microplate, and IFN in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Biotin labeled anti rat IFN was added; Polyclonal antibody, and IFN bound to the microplate; Binding, free components are washed away;

Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti rat IFN-γThe monoclonal antibody was coated on the microplate, and IFN-γ in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Biotin labeled anti rat IFN-γ was added; Polyclonal antibody, and IFN-γ bound to the microplate; Binding, free components are washed away; The streptavidin labeled horseradish peroxidase, which specifically recognizes biotin, is added to form a complex. After washing away the free components, the substrate solution (chromogenic agent) is added, and the color of the solution gradually turns blue. After adding the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Precoated 96 well plates, standards, anti rat IFN - γ One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 15.6 pg/ml

Detection Range: 39.1 - 2500 pg/ml

Recovery Range: 89.0-118.9%

Storage Method: 2-8 ℃ span>

Standard Curve

Background:

IFN-γ (interferon -γ)It is a typical proinflammatory cytokine produced by a variety of immune cells under inflammatory conditions, especially by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen-presenting molecules, and immunoglobulin class switching in B cells. It also has antiviral, antiproliferative and apoptotic effects. In addition, IFN-γ Acts as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. Ifn-γ Dimers pass through two ifn-γ R1 and two ifn-γ The receptor complex of the R2 subunit signals.

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SKU: 81588829602

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Primefan
Chelsea, US
★★★★★ 4
LOVE book...but a few swear words....
Format: Paperback
I LOVE this book! I love the plot! But, I would of reviewed this book 5 stars if: First, there are a few swear words throughout the book! As a kid, I find it disappointing to find a couple swear words in a kids book! The swear words, (a**, h***,d***) are used very loosely. I mean come on! Adding those swear words do NOT add to the plot and just make the book a 4 star review. Also, as a devoted Christian, I also find it disappointing that the characters say a couple of things ( God knows...., God....). If you are going to buy this book, I would make sure you're fine with a bit of language and some stuff......The plot is lovely, definitely a mystery book. The book definitely makes you think and get engaged. Finished book in 5 days and want the rest of the series. But if you buy this book, be careful of the swear words. I will read this book again....
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Reviewed in the United States on October 24, 2021
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Jeffrey Volk
Alexandria, US
★★★★★ 5
Good
Format: Hardcover
Good
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Reviewed in the United States on May 23, 2026
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Heidi Grange
Louisville, US
★★★★★ 5
Amusing and compelling.
Format: Paperback
Stuart Gibb has written a thoroughly engaging and amusing mystery. Setting this series inside an animal park was a brilliant move and it shows. Teddy makes a great detective as well as a smart aleck, Teddy proves to be determined and unwilling to give up. At first he wants the adults to investigate Henry's murder but when the police turn him away, his mother brushes him off, and the park itself covers it up, Teddy decides it's up to him to investigate. He recruits the help of Summer McCraken, the daughter of the owner of FunJungle and together they manage to wreak havoc as they search for the answers that nobody seems to want them to find. I found myself flying through this book once I got started. It's a great mystery with lots of suspects as well as twists and turns that made it unpredictable and delightful to read. I read this aloud to a bunch of classes and they all loved it. Note: there is a mild amount of what many would consider bad language.
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Reviewed in the United States on June 2, 2017
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E. Mullin
Lowell, US
★★★★★ 5
Hilarious and Heartfelt
Format: Hardcover
I started reading this to see if my cousins (who are between 7 and 11) would like it-- and I ended up loving it. It's a fun, mysterious, animal-friendly book. It not only keeps the reader on edge as an exciting whodunnit but also touches on deep questions of animal welfare and consumerism. The characters, both the children and the adults are touchingly real, filled with flaws and always given the chance to redeem themselves. Although it takes place in an imaginary zoo and much of the book is comedy, everything that happens feels so authentic. I'd recommend it to both kids and adults-- everyone will get something different out of it.
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Reviewed in the United States on May 25, 2010
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hannah grimsrud
Lexington, US
★★★★★ 5
This book kept me guessing and housing until the end, it deserves five stars!
Format: Kindle
I started to read this book because my friend was reading it on the way to swim practice and I wondered what kept her so intrigued and unresponsive. I asked her about it and she told me what it was about and what was currently happening. I was already rather to start it. I bought it and immediately began to read. I read for about four hours straight- stupidly- no joke!! It was one of few mystery/action-adventure novels that are actually really unpredictable in great ways! All I could think about was the story as I say in middle-school today, wondering what would happen next. I would most certainly recommend this to any reader, wether out not they like mystery or not!
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Reviewed in the United States on February 25, 2013

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