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Description
Human FS ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with follistatin (FS) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of follistatin (FS) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Follistatin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Follistatin (FS), also known as activin-binding protein, is encoded by the FS gene. Follistatin is an autocrine glycoprotein expressed in nearly all tissues of higher animals. It is produced by follicular stellate cells (FS) in the anterior pituitary gland. It is a member of the TGF-β superfamily. FS increases follistatin levels by increasing muscle mass in certain core muscle groups, thereby increasing life expectancy. The relationship between follistatin and polycystic ovary syndrome is also under investigation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.2 ★★★★★
Based on 790 reviews
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Product Reviews
★★★★★ 5
Decent Slim Fit Tux
Color: Midnight Black, Size: Large
This will work. In ordering the tux I don't recall seeing the set advertised as slim fit but it definitely is. My husband is 6'2" and 185 lbs. and is a runner. This suit just fits. The pants are thankfully long enough (he wears a 34/34) and the vest fits well however there doesn't seem to be an inch to spare in the jacket. He says it feels fine on, doesn't feel too snug, so that's all that matters. He needed a tux for a black tie wedding we'll be attending next month and this will fit the bill at a good price for something he'll likely never wear again. Note: the Suit comes folded up in a plastic bag and will need to be pressed before wearing.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 22, 2026
★★★★★ 5
Amazing suit and amazing price!
Color: Natural White, Size: X-Small, Color: Natural White, Size: X-Small
Can’t believe it was this cheap! Looked just like the suits friends were paying hundreds of $ for to not even buy but to rent. Would definitely recommend buying from here!
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Reviewed in the United States on May 5, 2026
★★★★★ 4
I'm going to be fair on this one
Color: Black, Size: XX-Large
TLDR; Ordered one (XL), too tight. Then ordered a size up (XXL), it was ok-ish but the sewing was horrible. Ended it up returning both and I still give them 4 stars (because this review is fair)
So to my review:
I wanted to like this suit. It had great reviews and it is, in all honesty, not that expensive. When I ordered the first suit (XL) I noticed that the fabric was super cheap but somewhat passable. The jacket did not close at all but the pants fit and they were surprisingly decent-ish. It is a slim fit suit so take it from there... the pants were tight in the leg BUT I did welcome A LOT the fact that comes with an elastic waist so it is pretty adjustable. THAT right there is probably one of my favorite things about this suit. I was on the fence of liking this suit so I thought maybe if I tried a bigger size it would made a difference.
So then I ordered an XXL and arrived the next day, great. Tried it and the jacket now fit but the pants were not the best fit of all... don't get me wrong, the elastic waist worked great and it was helpful but I definitely needed a belt for this one. Regardless, upon inspection of the new jacket I noticed that the one side was uneven. In the back, there is a separation between both sides, joined by a thin thread that joins both flaps together. You could tell that one side was longer than the other. I was upset but gave it some thought because this is what happens when you buy these type of things online, you are bound to find some items that may or may not work for you.
By the way, it comes with a nice vest (waaaaay too tight regardless the size) and a bowtie, which, in my opinion, just don't bother with this one guys
Truth be told, you can get a suit from a brand name retailer (on sale) for about $150- $200 that you might not encounter those QC issues but you might not have the same features as this one. Unfortunately, I don't have the time hoping the next one will be perfect.
It has it place in your life. If this is for a kid, prom, maybe a cheap wedding that you have to attend, it has its place given the price and features, but if you are going to use it for a gala event please don't, you will NOT look great.... that is why, even though I had to return both, I still gave it 4 stars and I will be buying a tux separates. More expensive, yes but it will also compliment my look completely. It won't be a one use only.
Hope this helps
P.S: Shipping has LOTS to be desired!! it comes in THE plastic bag that the suit comes folded in, nothing to protect it from the elements when its delivered, not even a shipping bag!!!. For that alone they will get a 1 Star (but that is on Amazon)
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Reviewed in the United States on April 28, 2023
★★★★★ 5
Symphony night
Color: Red, Size: Large, Color: Red, Size: Large
This Tuxedo fit just right on my husband. He wears a LG and I ordered a LG, perfect fit. Priced well.
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Reviewed in the United States on May 11, 2026
★★★★★ 5
Nice clean black, I did get the leg area tailored for his frame
Color: Black, Size: X-Small, Color: Black, Size: X-Small
Its worth the purchase. Purchased for prom and it ate!!! Good quality as well. Will purchase again.
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Reviewed in the United States on April 2, 2026
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