SKU: 53871864278

Biotinylated IgG1 Fc&Avi&His Tag Protein, Human

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Description

Biotinylated IgG1 Fc&Avi&His Tag Protein, HumanProduct Specification Species Human Synonyms IgG1 Fc, Ig gamma 1 chain C region, IGHG1 Accession P01857 1 Amino Acid Sequence Pro100 Lys330, with C Avi tag and 6*His and IgG1 Fc Expression System HEK293 Molecular Weight 33kDa (Reducing) Purity 95% by SDS PAGE Endotoxin <0. 1EU g Conjugation Biotin Tag Avi Tag, His Tag Physical Appearance Lyophilized Powder Storage Buffer PBS, pH7. 4. Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in

Product Specification


Species Human
Synonyms IgG1 Fc, Ig gamma-1 chain C region, IGHG1
Accession P01857-1
Amino Acid Sequence Pro100-Lys330, with C-Avi tag and 6*His and IgG1 Fc
Expression System HEK293
Molecular Weight 33kDa (Reducing)
Purity >95% by SDS-PAGE
Endotoxin <0.1EU/μg
Conjugation Biotin
Tag Avi Tag, His Tag
Physical Appearance Lyophilized Powder
Storage Buffer PBS, pH7.4.
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1. Peter Sondermann, Robert Huber, Vaughan Oosthuizen & Uwe Jacob: The 3.2-Å crystal structure of the human IgG1 Fc fragment–FcγRIII complex. Nature volume 406, pages267–273 (2000).

Background

Immunoglobulin G (IgG) is a monomer immunoglobulin, mainly involved in Immunoglobulin G (IgG) is a monomer immunoglobulin, mainly involved in secondary antibody reaction, plasma B cell synthesis and secretion, constitute 75% of human serum immunoglobulin. There are four subtypes of human IgG antibodies: IgG1, IgG2, IgG3, IgG4; mice IgG can be divided into five subtypes; IgG1, IgG2A, IgG2B, IgG2C and IgG3; rats have four subtypes: IgG1, IgG2A, IgG2B and IgG2C. The nomenclature of IgG subtypes of different species is independent, so there is no correlation among different genera. The content of IgG1 in serum accounts for about 60%, and the half-life in serum is about 21 days. After pepsin cleavage, IgG1 is divided into two antigenic binding sites F (ab) s and a highly conserved Fc fragment, and Fc has a highly conserved N-glycosylation site. IgG1 is the most potential subtype in tumor immunotherapy. The active form of IgG1 is bivalent monomer, and human IgG1 can also bind to mouse Fc γ receptor, so the obvious effect can be observed in mouse model.

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Exchange/Return Notes
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SKU: 53871864278

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Reviewed in the United States on March 22, 2026
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★★★★★ 5
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Good price, good quality
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Reviewed in the United States on May 16, 2026
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AMP
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Perfect for Volvo
This tool was perfect for removing the oil filter of my 2005 Volvo V70. It does what it’s supposed to do and was cheaper than the others. Very satisfied with this purchase.
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Reviewed in the United States on November 11, 2024
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★★★★★ 5
Does not work on BMW X3
too small, doesn't fit
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Reviewed in the United States on May 24, 2025
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Shawn D
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★★★★★ 4
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It’s alright. Does get stuck on the filter housing. Had to pry it off with a pry bar after I was done. But it did work
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Reviewed in the United States on October 20, 2024

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