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Description
Human IL-8/CXCL8 ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human IL 8 antibodies were pre coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL 8 present in the sample binds to the solid phase antibody and the
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-8 antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-8 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | 3-10C, AMCF-I, C-X-C motif chemokine 8, CXCL8, CXCL8SCYB8, Emoctakin, GCP1, GCP-1TSG-1, IL8, IL-8, interleukin 8, K60, LAI, LECT, MDNCF, MDNCFb-ENAP, member 8, MONAPGCP1, NAP1, NAP-1NAP1, NCF, Neutrophil-activating protein 1, Protein 3-10C, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1 | |||||||||||||||||||||||||||
| Composition |
Please use it within the validity period of the kit (new and old products are shipped randomly)
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| Background | Interleukin-8 (IL-8, also known as GCP-1, NAP-1, and CXCL8) is an 8-9kDa chemokine belonging to the α-type or CXC family that binds to heparin. So far, a total of 15 human CXC family proteins have been found, ranging in size from 8 to 12kDa. Most of them are located on human chromosome 4, all have a typical triple β-sheet layer/an α-helix structure, and most of them show Glu-Leu-Arg tripeptide motif at the N-terminus. Human IL-8 was first synthesized as a 99 amino acid precursor containing a 20 amino acid signal sequence and a 79 amino acid mature region. It can be circulated in vivo as monomers, homodimers, and heterodimers formed with CXCL4/PF4. IL-8 monomers are considered to be the most biologically active, whereas heterodimers may enhance the activity of PF4. At the amino acid level, mature human IL-8 has 65% and 70% homology to pigs and dogs, respectively. In rodents, the corresponding gene for IL-8 has not been found. A variety of IL-8 isoforms were generated by alternative splicing and differential proteolysis. Alternative splicing occurs at the C-terminus, where there is an 11 amino acid substitution (at aa # 92-99). Protease hydrolysis may be a cell-specific event that produces a truncation at the N-terminus of IL-8. For example, fibroblasts and vascular endothelial cells cleave amino acids 21 and 22 to form IL-8, while monocytes and lymphocytes cleave amino acids 21-25 to produce IL-8. These short IL-8s generally have higher biological activity, especially against the CXCR1IL-8 receptor. Approximately 15% of IL-8 is citrullinated at the precursor Arg27 position, which increases the half-life of IL-8 and promotes leukocytosis. Many types of cells secrete IL-8, including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II large alveolar cells, and endothelial cells. There are two IL-8 receptors, both of which belong to G protein-coupled receptor proteins: CXCR1/IL-8RA and CXCR2/IL-8RB, and the amino acid sequences between them share 77% homology. CXCR1 has a molecular weight of 45-50 kDa and is almost entirely exclusive to IL-8; CXCR2 has a molecular weight of 35-40 kDa and is common to all CXC chemokines. CXCR1 and CXCR2, respectively, form constitutive homodimers, which appear to be their functional configuration. Heterodimers also form when expressed in the same cell; But when it binds to IL-8, it will be disintegrated. CXCR2 responds to low concentrations of IL-8 and is mainly related to chemotaxis and MMP-9 release. In contrast, CXCR1 responds to high concentrations of IL-8 and is associated with respiratory bursts and activation of phospholipase D2. Therefore, in neutrophils CXCR2 is thought to direct the migration of neutrophils to the site of inflammation and then elicit CXCR1-mediated antibacterial activity. The best known function of IL-8 is its pro-inflammatory effect in immune cells. Essentially, IL-8 is secreted by a variety of cell types exposed to inflammatory stimulatory factors. For monocytes/macrophages, microbial exposure causes the release of IL-8; Subsequently, CXCR2-mediated chemotaxis migrates neutrophils to the site of antigen attack, accompanied by the activation and initiation of antibacterial activity in the next step. IL-8 can enhance the effect of M-CSF in bone marrow, thereby causing the maturation and release of granulocytes. These two functions of IL-8 complement each other. It has been reported that IL-8 has angiogenic function on tumor-associated endothelial cells. At this time, tumor-derived IL-8 can activate CXCR1 and CXCR2 in vascular endothelial cells in a paracrine manner. Both CXCR1 and CXCR2 are associated with the PI3-K/Akt and RasGTP signaling pathways and are involved in cell survival and proliferation. In addition, IL-8 positively regulates VEGFR2 and EGFR, which belong to receptor tyrosine kinases that mediate cell growth and migration. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Kit unopened, stored at 2-8 ℃. | |||||||||||||||||||||||||||
| Test Range | 31.3pg/mL-2000pg/mL |
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4.4 ★★★★★
Based on 29 reviews
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Product Reviews
★★★★★ 5
They prevent leaks, and fit great!
We have always used Huggies diapers for our babies and young toddlers because they were the only ones that never leaked or caused a rash. My second daughter was fully potty trained at 2.5 years old and never needed pull-ups. However, our third daughter, who also trained at 2.5 years old, digressed with her nighttime potty training due to instability - we moved twice within the first 6 weeks after she potty trained. So we had to get pull-ups for her to wear at night. Obviously since we loved Huggies diapers, we tried Huggies Pull-Ups, and again, they did not disappoint! They never leak, even on nights when she doesn't get up to go at all, and has lots of pee, and they have never caused a rash either, no matter how full. She's been using them for 1.5 years, at night, and we have never had any issues. They are dependable: decently comfortable to the child, do not cause rashes, fit properly with the ability to adjust at the thigh (if needed), and they prevent leaks! I highly recommend them!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 19, 2025
★★★★★ 5
Price good
Size: 5T-6T, Unit Count: 80
Perfect for starters
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 11, 2026
★★★★★ 5
Great fit and makes potty training easier
Size: 3T-4T, Unit Count: 84
These training pants have been a big help during potty training. They fit comfortably and feel soft against the skin, more like real underwear than a diaper. The stretchy sides make them easy for little ones to pull up and down on their own, which really helps with independence.
They do a good job holding small accidents without leaking, and the fun character designs make my little one more excited to wear them. The pack comes with plenty to last through the training phase, which is nice when you’re going through a few each day.
Tip: keep a few extras in the car or diaper bag for outings. It makes potty training on the go so much easier.
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Reviewed in the United States on October 14, 2025
★★★★★ 5
Great Pull-ups that Don't Leak!
Size: 3T-4T, Unit Count: 20
I was on the hunt for the perfect potty training pants for my little guy, and Pull-Ups Boys' Potty Training Pants in size 3T-4T turned out to be a great find. We’ve tried a few different brands, but these have been the most reliable. The design is fun and colorful, which my son loves, and it makes him excited to wear them. The fit is spot on for his size (32-40 lbs), and he moves around comfortably without any leaks. The absorbency is impressive – it gives me peace of mind knowing accidents won’t mean a complete outfit change. Overall, these Pull-Ups have made the potty training process a lot smoother for both of us.
There are a few things to note, though. The price is a bit higher compared to some other brands, but I believe the quality justifies it. Also, while they are great during the day, we still need a bit more protection at night. I find myself using an extra layer for nighttime just to be safe. The packaging could also be improved – a resealable option would be nice to keep the remaining training pants fresh and organized. Despite these minor issues, I’m really happy with the product, and my son is well on his way to being fully potty trained.
Pros:
- Fun and colorful design that my son loves
- Perfect fit for 32-40 lbs, allowing comfortable movement
- Excellent absorbency, minimizing leaks
- Helps make the potty training process smoother
Cons:
- Slightly higher price compared to other brands
- Not as effective for overnight use, requiring additional protection
- Packaging lacks a resealable option for convenience
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Reviewed in the United States on June 5, 2024
★★★★★ 5
Leak proof training pants
Size: 3T-4T, Unit Count: 112
These training pants are amazing. My son uses them whenever he’s taking a nap or sleeping throughout the night. I’ve ordered these multiple times and we’ve never had an issue with leaking.
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Reviewed in the United States on May 19, 2026
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