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Description
Human IL-12 p70 ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human IL 12 p70 antibodies were pre coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, IL 12 p70 present in the sample binds to the solid phase antibody
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-12 p70 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, IL-12 p70 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | Human interleukin 12p70 detection kit, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NKSF1, p35 | |||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
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| Background | Interleukin 12 p70 (IL-12), also known as natural killer cell stimulating factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine consisting of a 40kDa (p40) subunit and a 35kDa (p35) subunit. Human p40 may circulate as a monomer, a homodimer, or a complex that binds to p35 to form IL-12 or binds to p19 to form IL-23. Both homodimers p40 and IL-23 can bind to IL-12R as non-signal antagonists. Alternatively, p40 can also bind to Rβ1, activating microglia and macrophages. The IL-12 p40 subunit shares with another heterodimeric cytokine, IL-23, with similar and distinct biological activity from IL-12. Cells known to produce IL-12 include macrophages and dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, plasmacytoid dendritic cells, microglia, CD8 + DCs (mouse cells only), and non-germinal center (CD38-CD44 +) B cells (human cells only). Functional IL-12 was shown to enhance cytotoxic activity and induce interferon gamma (IFN-gamma) production within NK cells, T cells, and dendritic epidermal T cells. IL12 has also been reported to induce IFN-γ production by macrophages. IL-12, together with other members of the IL-12 family, IL-23 and IL-27, promotes the development of CD4+Th1 immune response. In response to infection, IL-27 is first released, promoting the transition from Th0 to Th0/1. IL-12 is subsequently produced, resulting in Th1 effector cells. IL-12 combines with IL-18 to generate Th1 memory cells from effector cells, which are subsequently activated by IL-23. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 31.3pg/mL-2000pg/mL |
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Product Reviews
★★★★★ 5
A great sport visor
Size: One Size, Color: (100) White / White / Reflective
My head is small so a lot of visors don’t fit because of the Velcro. This is perfect and the ability to wet it for coolness is great. Love it for tennis.
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Reviewed in the United States on August 14, 2025
★★★★★ 5
Great for running
Size: One Size, Color: (001) Black / Black / Reflective
Hat fits well and worked great for my marathon. The Velcro doesn’t pull on my hair.
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Reviewed in the United States on November 24, 2025
★★★★★ 5
Cute and comfortable, though a little big without tightening a bit
Size: One Size, Color: (100) White / White / Reflective
Love the bright color and material. It fits a little big at first but has the ability to tighten quite a bit so it should be fine like it is for me. I just have a smaller head. It has great coverage. Feels comfortable as well.
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Reviewed in the United States on January 7, 2026
★★★★★ 4
Perfect fit
Size: One Size, Color: (001) Black / Black / Reflective
I ordered this to wear during my upcoming vacation. It will shade my face from direct sunlight. I initially wished the brim was just a tad bit shorter but then I realize the current brim size will serve the purpose; that being the reason why it was purchased.
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Reviewed in the United States on June 24, 2025
★★★★★ 5
Buy it!
Size: One Size, Color: (001) Black / Black / Reflective, Size: One Size, Color: (001) Black / Black / Reflective
This hat (visor) is by far one of the most comfortable ones I’ve ever worn! Absolutely love it!!!!
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Reviewed in the United States on May 18, 2026