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Description
Mitochondrial Membrane Potential Detection Kit (jc-10)Product Specification Usage 1. Preparation of JC 10 dyeing working solution: The amount of JC 10 staining working solution required for each well of the six well plate is 1 mL, and the amount of JC 10 staining working solution for other culture vessels is similar; For the cell suspension, 0. 5 mL of JC 10 staining working solution is required for every 500,000 to 1,000,000 cells. Take an appropriate amount of JC 10 (200 ) and dilute JC 10 in the
Product Specification
| Usage | 1. Preparation of JC-10 dyeing working solution: The amount of JC-10 staining working solution required for each well of the six-well plate is 1 mL, and the amount of JC-10 staining working solution for other culture vessels is similar; For the cell suspension, 0.5 mL of JC-10 staining working solution is required for every 500,000 to 1,000,000 cells. Take an appropriate amount of JC-10 (200 ×) and dilute JC-10 in the proportion of 8mL of ultrapure water per 50μL of JC-10 (200 ×). Thoroughly dissolve and mix JC-10 with vigorous shaking. Then add 2mL of JC-10 staining buffer (5 ×), and mix well to obtain JC-10 staining working solution. 2. Setting of positive control: CCCP (10 mM) provided in the kit is recommended to be added to the cell culture medium at a ratio of 1: 1000, diluted to 10 μM, and treated the cells for 20 minutes. Subsequently, JC-10 was loaded according to the following method, and mitochondrial membrane potential was detected. For most cells, the membrane potential of mitochondria will usually be completely lost after 20 minutes of 10 μM CCCP treatment, and it should show green fluorescence after JC-10 staining; However, normal cells should show red fluorescence after staining with JC-10. For specific cells, the concentration and duration of CCCP may be different, and it is necessary to refer to relevant literature to decide. 3. For suspension cells: (1) Take 100,000 to 600,000 cells and resuspend them in 0.5 mL cell culture medium. The cell culture medium can contain serum and phenol red. (2) Add 0.5 mL of JC-10 dyeing working solution, reverse it several times and mix well. Incubate in a cell incubator at 37 °C for 20 minutes. (3) During the incubation period, an appropriate amount of JC-10 staining buffer (1 ×) was prepared according to the ratio of adding 4 mL of distilled water per 1 mL of JC-10 staining buffer (5 ×), and placed in an ice bath. (4) After the incubation at 37 °C, 600g was centrifuged at 4 °C for 3-4 minutes to precipitate the cells. Discard the supernatant and be careful not to aspirate the cells as much as possible. (5) Wash twice with JC-10 staining buffer (1 ×): add 1 mL of JC-10 staining buffer (1 ×) to resuspend the cells, centrifuge 600 g at 4 °C for 3-4 minutes, pellet the cells, and discard the supernatant. Add 1 mL of JC-10 staining buffer (1 ×) to resuspend the cells, centrifuge 600g at 4 °C for 3-4 minutes, pellet the cells, and discard the supernatant. (6) After resuspending with an appropriate amount of JC-10 staining buffer (1 ×), observe with a fluorescence microscope or laser confocal microscope, or detect with a fluorescence spectrophotometer or analyze with a flow cytometer. 4. For adherent cells: Note: For adherent cells, if you want to use fluorescence spectrophotometer or flow cytometer detection, you can collect the cells first, and refer to the detection method of suspended cells after resuspension. (1) For one well of a six-well plate, aspirate the culture medium, wash the cells once with PBS or other appropriate solution if necessary according to the specific experiment, and add 1 mL of cell culture medium. The cell culture medium may contain serum and phenol red. (2) Add 1mL of JC-10 dyeing working solution and mix well. Incubate in a cell incubator at 37 °C for 20 minutes. (3) During the incubation period, an appropriate amount of JC-10 staining buffer (1 ×) was prepared according to the ratio of adding 4 mL of distilled water per 1 mL of JC-10 staining buffer (5 ×), and placed in an ice bath. (4) After the incubation at 37 °C, the supernatant was aspirated and washed twice with JC-10 staining buffer (1 ×). (5) 2 mL of cell culture medium is added, and the culture medium may contain serum and phenol red. (6) Observe under fluorescence microscope or laser confocal microscope. 5. For purified mitochondria: (1) The prepared JC-10 staining working solution was diluted 5 times with JC-10 staining buffer (1 ×). (2) 0.9 mL of 5-fold diluted JC-10 staining working solution was added with 0.1 mL of purified mitochondria with a total protein amount of 10-100 μg. (3) Detection with a fluorescence spectrophotometer or a fluorescence microplate reader: After mixing, directly use a fluorescence spectrophotometer to time scan (time scan). The excitation wavelength is 485nm and the emission wavelength is 590nm. If a fluorescent microplate reader is used and the excitation wavelength cannot be set to 485 nm, the excitation wavelength can be set in the range of 475 to 520 nm. In addition, fluorescence detection can also be performed with reference to the wavelength setting in step 6 below. (4) Observation with fluorescence microscope or laser confocal microscope: The method is the same as step 6 below. 6. Fluorescence observation and result analysis: When detecting JC-10 monomer, the excitation light can be set to 490nm and the emission light can be set to 530nm; When detecting the JC-10 polymer, the excitation light can be set to 525 nm and the emission light can be set to 590 nm. Note that it is not necessary to set the excitation light and the emission light at the maximum excitation wavelength and the maximum emission wavelength when measuring fluorescence here. For observation using a fluorescence microscope, when detecting JC-10 monomer, you can refer to the settings when observing other green fluorescence, such as the settings when observing GFP or FITC; When detecting JC-10 polymer, you can refer to the settings when observing other red fluorescence, such as propidium iodide or Cy3. The appearance of green fluorescence indicates that the mitochondrial membrane potential decreases, and the cell is probably in the early stage of apoptosis. The appearance of red fluorescence indicates that the mitochondrial membrane potential is relatively normal and the state of cells is also relatively normal. Precautions: 1. JC-10 (200 ×) will solidify and stick to the bottom, wall or cover of the centrifuge tube at lower temperatures such as 4 °C and ice bath. It can be incubated in a water bath at 20 ~ 25 °C for a while until it is completely melted. After use. 2. JC-10 (200 ×) must be fully dissolved and mixed with the ultrapure water provided by the kit before JC-10 staining buffer (5 ×) can be added. Do not prepare JC-10 staining buffer (1 ×) first and then add JC-10 (200 ×), as it will be difficult to fully dissolve JC-10, which will seriously affect subsequent detection. 3. When washing with JC-10 staining buffer (1 ×) after loading JC-10, keep the JC-10 staining buffer (1 ×) at about 4 ℃, and the washing effect at this time is better. 4. After the JC-10 probe is loaded and washed, try to complete the follow-up detection within 30 minutes. Store in ice bath before testing. 5. Please do not prepare all JC-10 staining buffer (5 ×) into JC-10 staining buffer (1 ×). JC-10 staining buffer (5 ×) should be directly used during the use of this kit. 6. If precipitate is found in JC-10 staining buffer (5 ×), it must be completely dissolved before use. To promote dissolution, it can be heated at 37 °C. 7. CCCP is a mitochondrial electron transport chain inhibitor, which is toxic. Please pay attention to careful protection. 8. For your safety and health, please wear a lab coat and disposable gloves. |
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| Beilstein | 0 | ||||||||||
| Description |
Mitochondrial membrane potential detection kit (JC-10) is a kit that uses JC-10 as a fluorescent probe to rapidly and sensitively detect the changes of mitochondrial membrane potential of cells, tissues or purified mitochondria. It can be used for early apoptosis detection.
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| PubChem CID | 0 | ||||||||||
| Storage Temp. | Dry and protected from light at-20 ℃, shelf life is 12 months. Ultrapure water and JC-10 staining buffer (5 ×) can also be stored at 4 °C. |
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4.3 ★★★★★
Based on 29 reviews
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Product Reviews
★★★★★ 5
Typhur Sync Gold Wireless Meat Thermometer
Color: Black
I’ve been extremely impressed with the Typhur Sync Gold Wireless Meat Thermometer. From the first use, it’s clear this is a premium product designed with both convenience and accuracy in mind. The wireless setup makes cooking so much easier—no more dealing with tangled cords or constantly opening the oven or grill to check temperatures. The app is intuitive and provides real-time updates, which gives me total confidence that my food is cooking exactly the way I want it.
What really sets this product apart, though, is not just the performance—it’s the exceptional customer service. I ran into an issue with one of the probes, and I have to say their support team was outstanding. They responded quickly, were easy to work with, and resolved the problem without any hassle. They sent me a replacement probe at no cost, which really shows they stand behind their product and care about their customers.
Overall, the Typhur Sync Gold delivers on all fronts: accuracy, ease of use, and reliability. Add in their top-notch customer support, and it’s an easy recommendation for anyone serious about cooking or grilling.
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Reviewed in the United States on May 29, 2026
★★★★★ 5
Best wireless thermometer (and we've had a bunch)
Color: Black, Color: Black
It's dead accurate and simple to use. Meat temp and grill/oven temp in one probe isn't new but it works perfectly on the Typhur. Temps show immediately when the probe is removed and we haven't had any connection issues. Wifi and the 10x sub-1g is so much better than bluetooth. We can have the grill outside the walkout patio and the base unit connects everywhere in our house.
The Typhur app is full featured, polished and easy to use. One app works with their oven, air fryers and sous vide machine. The price is too low to believe. We bought two of these and will be throwing our other wireless unit away.
Hoping to try other Typhur products in the future.
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Reviewed in the United States on November 29, 2025
★★★★★ 5
A Game-Changer for Precision Cooking
Color: Black
We’ve had the Typhur Sync Smart Meat Thermometer for a few months now, and it’s been nothing short of amazing. The wireless probe is a total game-changer, it frees you up from cords and gives you the flexibility to monitor your cook remotely. It charges via USB-C, which is super convenient. I can use the same charger that powers my smartphone to charge a meat thermometer, imagine saying that sentence 20 years ago.
The companion app is one of the highlights. It displays incredibly useful temperature data, including readings from multiple points within the probe to show the gradient of heat inside the meat. You even get a real-time graph tracking the temperature over time, which is especially helpful for monitoring the entire cooking process.
And best of all: the app is currently free with no subscription required (fingers crossed it stays that way).
The thermometer seems to be very accurate. While I haven’t tested it against lab equipment, the NIST certification and consistently reliable results give me confidence. I’ve made sure the temps fall within my personal margin of error—and so far, so good. I can’t speak for others who may have had issues with the probe, but from what I’ve seen, Typhur’s customer service has a solid reputation. I appreciate that they seem to genuinely stand behind their products.
While I primarily use the thermometer for reverse searing steaks, it’s versatile enough for anything from steak to turkey, chicken, or pork loin. If you’re serious about cooking and want precision without hassle, this is a fantastic investment.
Highly recommended.
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Reviewed in the United States on June 4, 2025
★★★★★ 5
This thing no longer working. Too late for a refund so the loss is on us. Buyer beware.
Color: Black
We wanted a temperature probe that was simple and works well. This one was it. We grill as often as possible and were just tired of having a good piece of meat come out either over or under cooked. I like a steak medium and my wife likes it medium well to well done. We use a Weber Genesis E410 that works great and has good heat control. Our first use of this Typhur thermometer gave us a great result. Not sure how it will work on a larger item like a brisket or a turkey but, at this point, we see no reason to expect any lesser result. It had accuracy, good range, and seems quite durable. Grilling is, among other things, is its own social event., so we did not use an "Ap" to watch the event on a smart phone. (We try to limit the use of smart phones when we are outside to encourage human interaction.) Typhur offers an Ap but the base unit was all we wanted to try and it works great.
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Reviewed in the United States on October 8, 2025
★★★★★ 4
Accurate and affordable
Color: Black
Four months after I purchased the thermometer, I have to say it is the best probe thermometer I have used. The temperature reading are accurate and the unit functions well. Very easy to use with or without the app. The display is easy to read but the app allows you to see all six temperature zones! Catch it on sale to get the best price. The probe battery only lasted six hours so I notified Typhur and they are replacing it without question! I purchased a second unit for a Christmas gift.
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Reviewed in the United States on February 5, 2026