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Description
Rat Clusterin ELISAThe Rat Clusterin ELISA is an enzyme immunoassay for the quantitative determination of Clusterin in rat serum and rat urine. In the Rat Clusterin ELISA kit, standards and samples are incubated in microtitration wells pre coated with polyclonal anti rat clusterin antibody. After 60 minutes incubation followed by washing, biotin labelled polyclonal anti rat clusterin antibody is added and incubated with the captured clusterin for 60 minutes. After
The Rat Clusterin ELISA is an enzyme immunoassay for the quantitative determination of Clusterin in rat serum and rat urine.
In the Rat Clusterin ELISA kit, standards and samples are incubated in microtitration wells pre-coated with polyclonal anti-rat clusterin antibody. After 60 minutes incubation followed by washing, biotin-labelled polyclonal anti-rat clusterin antibody is added and incubated with the captured clusterin for 60 minutes. After another washing, streptavidin-HRP conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of rat Ccusterin. A standard curve is constructed by plotting absorbance values against clusterin concentrations of standards and concentrations of unknown samples are determined using this standard curve.
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