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Description
Rat THBS4 (Thrombospondin-4) ELISA KitThe Rat (THBS4) Thrombospondin 4 ELISA Kit measures Thrombospondin 4 in Rat samples. The plate has been pre coated with Rat THBS4 antibody. THBS4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat THBS4 Antibody is added and binds to THBS4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated THBS4 antibody. After incubation unbound Streptavidin HRP is washed away during a
The Rat (THBS4) Thrombospondin-4 ELISA Kit measures Thrombospondin-4 in Rat samples. The plate has been pre-coated with Rat THBS4 antibody. THBS4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat THBS4 Antibody is added and binds to THBS4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated THBS4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat THBS4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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