SKU: 18143208567

Human CD30L (Cluster of Differentiation 30 Ligand) ELISA Kit

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Description

Human CD30L (Cluster of Differentiation 30 Ligand) ELISA KitThe Human CD30L (Cluster of Differentiation 30 Ligand) ELISA Kit measures CD30L in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CD30L antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human CD30L (Cluster of Differentiation 30 Ligand) ELISA Kit measures CD30L in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CD30L antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CD30L bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CD30L in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 18143208567

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4.4 ★★★★★
Based on 707 reviews
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Christy D.
Alexandria, US
★★★★★ 5
Aggressive chewer who loves fetch approved!!
Size: Medium, Size: Medium
I highly recommend the Chuck-It brand in general, especially for dogs with an aggressive bite. These balls are awesome and are the most durable balls I’ve bought for my 50 lbs lab mix. He has not been able to break one of them yet and we’ve had a few of the squeaky balls for months. They are a bit pricey but worth it. The one in the pic has been used for months, lives outside and you can see it’s still in great shape.
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Reviewed in the United States on June 22, 2025
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Verified Purchase
Tasha U
Dallas, US
★★★★★ 5
Good quality dog ball for playing fetch
Size: Medium
My dog and family love these chuck it balls to play fetch with our dog. They are durable and fun. They squeak which is added fun for any dog! Regular tennis balls my dog chews apart and they get pretty gross after playing and being left outside. These can be easily washed off and cleaned. These balls last! Would recommend to anyone with a dog and would purchase again.
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Reviewed in the United States on April 17, 2025
R
Verified Purchase
ryan johnson
Lexington, US
★★★★★ 5
Tough and sturdy
Size: Medium
Have lasted my dog for years now and she is a tough chewer. Worth the money
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Reviewed in the United States on December 12, 2025
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Verified Purchase
Lorraine Nathanson
Whiting, US
★★★★★ 5
Colorful, Sturdy & Great Value
Color: Large Set of 4
Purchased for our two puppies when they were 2 months old and weighed about 14 pounds so theses balls were a little big for them to hold in their mouths but that didn't stop them from trying. While they did like these balls they much preferred softer soccer balls, the later of which did not hold up well at all. I had hoped they would play with these balls more when they were older but that hasn't happened 5 months later. I guess it's no fun if you can't tear the toy apart. Colors are very bright and they do enjoy chasing after them as long as I am the one rolling them across the room for them. Maybe when they progress to batting things with their paws they will start to do this for themselves.
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Reviewed in the United States on May 18, 2025
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Verified Purchase
Erik
San Leandro, US
★★★★★ 5
My pup loves them
Color: Large Set of 4
Colors are bright and my dog loves the squeaky! Good size no worries of choking.
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Reviewed in the United States on September 10, 2025

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